Difference between revisions of "Part:BBa K1100010"
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A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015).
 
A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015).
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The generator is designed to express the Csy4 protein for a futher usage for the cleavage to the csy4 insulator.
+
The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator.
 
Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.
 
Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Expression of the Csy4 gene.
  
 
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Revision as of 16:22, 27 September 2013

Csy4 generator (with terminator)

A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015). The generator is designed to express the Csy4 for a futher usage for the cleavage to the csy4 insulator. Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164