Difference between revisions of "Part:BBa K1024001:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | + | We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcriptional regulated promoter in quorum sensing system, Plux promoter (BBa_R0062), by adding cyc100 mini promoter downstream of the Plux promoter (BBa_K1024001). | |
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===Source=== | ===Source=== | ||
Latest revision as of 16:19, 27 September 2013
Lux Promoter in Yeast (pLux+Cyc Promoter+mCherry)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 159
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcriptional regulated promoter in quorum sensing system, Plux promoter (BBa_R0062), by adding cyc100 mini promoter downstream of the Plux promoter (BBa_K1024001).
Source
S. cerevisiae & Registry
References
[1]Fuqua W C, Winans S C, Greenberg E P. Quorum sensing in bacteria: the LuxR-LuxI family of cell density-responsive transcriptional regulators[J]. Journal of bacteriology, 1994, 176(2): 269.
[2]Sadowski I, Ma J, Triezenberg S, et al. GAL4-VP16 is an unusually potent transcriptional activator[J]. Nature, 1988, 335(6190): 563-564.