Difference between revisions of "Part:BBa K1100010"

 
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__NOTOC__
 
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<partinfo>BBa_K1100010 short</partinfo>
 
<partinfo>BBa_K1100010 short</partinfo>
  
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R0040-csy4 insulator-B0034-Csy4-B0015
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A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015).
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The generator is designed to express the Csy4 protein for a futher usage for the cleavage to the csy4 insulator.
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Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:13, 27 September 2013

Csy4 generator (with terminator)

A seious of parts are put together onto the pSB1C3 plasmid including the promter(BBa_R0040),csy4 insulator(16-nt), a RBS(BBa_B0034), the Csy4 gene and the teminator (BBa_B0015). The generator is designed to express the Csy4 protein for a futher usage for the cleavage to the csy4 insulator. Meanwhile, it has a insulator itself to avoid the disturbance between the patterns.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 164