Difference between revisions of "Part:BBa K1024003"

 
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<partinfo>BBa_K1024003 short</partinfo>
 
<partinfo>BBa_K1024003 short</partinfo>
  
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<p>
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<b>Part Name:</b> BBa_K1024003
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<b>Short Description:</b> Reporter for Tet system in Yeast
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<b>Part Type:</b> Signaling
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<b>Design:</b> The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype.
  
 
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Revision as of 16:12, 27 September 2013

Reporter for Tet system in Yeast

Part Name: BBa_K1024003

Short Description: Reporter for Tet system in Yeast

Part Type: Signaling

Design: The part is designed to valid the Tet system in yeast. TetR gene with VP16 is constitutively expressed, activating the downstream reporter gene of TetO and CYC1 TATA region. In the yeast Saccharomyces cerevisiae the ade2, and/or the ade1, mutation in the adenine biosynthetic pathway leads to the accumulation of a cell-limited red pigment. Thus, it could be used as a marker for screening of target phenotype. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3077
    Illegal BamHI site found at 2480
    Illegal XhoI site found at 1779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]