Difference between revisions of "Part:BBa K1024000"

Revision as of 15:58, 27 September 2013

BBa_K1024000

LuxR in Yeast (pTEF+VP16+NLS+LuxR)

Part Name: BBa_K1024000

Short Description: LuxR in Yeast (pTEF+VP16+NLS+LuxR)

Part Type: Regulatory

Design: We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcription activator, LuxR (BBa_C0062), by adding nuclear localization signal (NLS) sequence and Herpes simplex virus VP16 activation domain in N-terminus of LuxR, and ligate the sequence of this modified LuxR downstream TEF promoter, which is the constitutive promoter in yeast (BBa_K1024000).

Usage and Biology

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1580
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321

@@ Line 1: / Line 1: @@
+<h2>BBa_K1024000</h2>
 __NOTOC__
 <partinfo>BBa_K1024000 short</partinfo>
-<b>TEF+VP16+LuxR</b>
+<p>
+					<b>Part Name:</b> BBa_K1024000
-<!-- Add more about the biology of this part here
+				</p>
+				<p>
+					<b>Short Description:</b> LuxR in Yeast (pTEF+VP16+NLS+LuxR)
+				</p>
+				<p>
+					<b>Part Type:</b> Regulatory
+				</p>
+				<p>
+					<b>Design:</b> We constructed and improved standard BioBrick Parts about quorum sensing systems and modified the systems in prokaryotic microorganisms for usage in S. cerevisiae (Yeast). We modified the transcription activator, LuxR (BBa_C0062), by adding nuclear localization signal (NLS) sequence and Herpes simplex virus VP16 activation domain in N-terminus of LuxR, and ligate the sequence of this modified LuxR downstream TEF promoter, which is the constitutive promoter in yeast (BBa_K1024000).
 ===Usage and Biology===