Difference between revisions of "Part:BBa K1100012"

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	<partinfo>BBa_K1100012 short</partinfo>		<partinfo>BBa_K1100012 short</partinfo>
		+	<p></p>The RNAINS1 comes from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.
		+	Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator BBa_R1051
		+	[[File:RNA-IN-RNA-OUT.jpg|800px|thumb|center|Figure1. mechanism of RNA-INOUT regulatory system]]
		+	[[File:INS1.jpg|400px|thumb|center|Figure2. predicted structure of RNAINS1]]
−	coming soon	+	References:
		+	<p></p>Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.
	<!-- Add more about the biology of this part here		<!-- Add more about the biology of this part here

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Revision as of 15:51, 27 September 2013

OUTS1 repressible cI

The RNAINS1 comes from a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. The translation repression rate between RNAOUTS1 and RNAIN S1 is 90%.

Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator BBa_R1051

References:

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features