Difference between revisions of "Part:BBa K1188002"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1188002 short</partinfo> | <partinfo>BBa_K1188002 short</partinfo> | ||
RTX (repeats-in-toxin) is a polypeptide motif consisting of a repeating sequence of amino acids. It undergoes a conformation change in the presence of calcium which causes it to precipitate from solution. This is the shorter (8-mer) version of this part, and is in Freiburg format. This allows RTX to be fused to itself to create a longer RTX chain, or to other proteins which can then be precipitated from a solution. This is useful for the purpose of protein purification, or alternatively to remove an unwanted protein from a solution. | RTX (repeats-in-toxin) is a polypeptide motif consisting of a repeating sequence of amino acids. It undergoes a conformation change in the presence of calcium which causes it to precipitate from solution. This is the shorter (8-mer) version of this part, and is in Freiburg format. This allows RTX to be fused to itself to create a longer RTX chain, or to other proteins which can then be precipitated from a solution. This is useful for the purpose of protein purification, or alternatively to remove an unwanted protein from a solution. | ||
| + | |||
| + | Experimentally results show that calcium concentrations of 10 mM or above results in effective precipitation while conserving the function of the purified protein, in the the case of GFP. Calcium concentrations below ~1 mM do not produce a useful amount of precipitate. | ||
| + | |||
| + | [[File:RTXPrecipitation.PNG|200px]] | ||
| + | [[File:RTXSDSPage.PNG|200px]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 15:24, 27 September 2013
RTX - Calcium Binding Protein
RTX (repeats-in-toxin) is a polypeptide motif consisting of a repeating sequence of amino acids. It undergoes a conformation change in the presence of calcium which causes it to precipitate from solution. This is the shorter (8-mer) version of this part, and is in Freiburg format. This allows RTX to be fused to itself to create a longer RTX chain, or to other proteins which can then be precipitated from a solution. This is useful for the purpose of protein purification, or alternatively to remove an unwanted protein from a solution.
Experimentally results show that calcium concentrations of 10 mM or above results in effective precipitation while conserving the function of the purified protein, in the the case of GFP. Calcium concentrations below ~1 mM do not produce a useful amount of precipitate.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 223 - 1000COMPATIBLE WITH RFC[1000]