Difference between revisions of "Part:BBa K1124008"

 
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anti-hycA sRNA
 
anti-hycA sRNA
  
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===Usage and Biology===
 
===Usage and Biology===
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'''The Effects on Hydrogen Production of HycA'''
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Hydrogen production in ''Escherichia coli'' is performed by Formate Hydrogen Lyase complex(FHL complex), which catabolizes formate into hydrogen and carbon dioxide. FHL complex consists of proteins named HycB, HycC, HycD, HycE, HycF, HycG, and FdhF. Most of these proteins other than FdhF are coded in ''hyc'' operon, which is transcriptionally activated and inhibited by FhlA([[Part:BBa K778001]], [[Part:BBa K778002]]) and HycA, respectively. ''fhlA'' overexpression is known to increase hydrogen production in ''E. coli'', so we expects that repression of ''hycA'' expression will also lead to hydrogen production increasing.
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'''RNA Silencing Utilizing MicC sRNA Scaffold'''
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The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold([[BBa_K1124005]]) works as a tool of RNA silencing (see descriptions in anti-hycA sRNA). In this part,anti-hycA sRNA, the target-binding sequence is complementary to the first 24bp of hycA CDS(that is, the sequence from start codon ATG and the following 7 triplet codons).
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We expect that this part will repress ''hycA'' expression in post-transcriptional way, and that expressing this part under proper promoters may lead to hydrogen production increase.
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1124008 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1124008 SequenceAndFeatures</partinfo>

Revision as of 13:32, 27 September 2013

anti-hycA sRNA

anti-hycA sRNA

Usage and Biology

The Effects on Hydrogen Production of HycA

Hydrogen production in Escherichia coli is performed by Formate Hydrogen Lyase complex(FHL complex), which catabolizes formate into hydrogen and carbon dioxide. FHL complex consists of proteins named HycB, HycC, HycD, HycE, HycF, HycG, and FdhF. Most of these proteins other than FdhF are coded in hyc operon, which is transcriptionally activated and inhibited by FhlA(Part:BBa K778001, Part:BBa K778002) and HycA, respectively. fhlA overexpression is known to increase hydrogen production in E. coli, so we expects that repression of hycA expression will also lead to hydrogen production increasing.


RNA Silencing Utilizing MicC sRNA Scaffold

The combination of the target-binding sequence of a gene to be repressed with MicC sRNA scaffold(BBa_K1124005) works as a tool of RNA silencing (see descriptions in anti-hycA sRNA). In this part,anti-hycA sRNA, the target-binding sequence is complementary to the first 24bp of hycA CDS(that is, the sequence from start codon ATG and the following 7 triplet codons). We expect that this part will repress hycA expression in post-transcriptional way, and that expressing this part under proper promoters may lead to hydrogen production increase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]