Difference between revisions of "Part:BBa K1149050"
Line 3: Line 3:
  
 
This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly.  
 
This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly.  
 +
There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD.
  
 
http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg
 
http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg

Revision as of 13:25, 27 September 2013

Bdh2 (intracellular)

This part expresses Bdh2 3HB dehydrogenase enzyme intracellularly. There is a GFP in operon with the enzyme which can be used to monitor expression levels. The promoter is arabinose inducible pBAD.

http://www.igem.org/wiki/images/f/f0/Reaction_bdh.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 181
    Illegal AgeI site found at 1126
  • 1000
    COMPATIBLE WITH RFC[1000]