Difference between revisions of "Part:BBa K1074006"
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This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method. | This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method. | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | ===References=== | ||
+ | SUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4 | ||
+ | |||
+ | 1.张灏,夏雨,傅晓燕等.耐热β2半乳糖苷酶基因bgaB 在枯草芽孢杆菌中的整合表达.Journal of Wuxi University of Light Industry 第22卷第6期.2003年11月. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 13:22, 27 September 2013
Pgrac+RBS+SamyQ+TD1+GFP
This is the main circuit of our project to allow high expression of target protein(antigen,adjuvant).GFP can be substituted by various proteins via a modular PCR or standard cut/ligation method.
Usage and Biology
References
SUN Zheng, WEN Long-ping.Transdermal delivery of fusion green fluorescent protein mediated by covalently associated TD1 peptide. OURNAL OF UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA.2009.Vol.39,No.4
1.张灏,夏雨,傅晓燕等.耐热β2半乳糖苷酶基因bgaB 在枯草芽孢杆菌中的整合表达.Journal of Wuxi University of Light Industry 第22卷第6期.2003年11月. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 984