Difference between revisions of "Part:BBa K1074006:Experience"

(→‎User Reviews)
(→‎User Reviews)
Line 28: Line 28:
 
In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant
 
In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant
 
Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA.
 
Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA.
−
[[Image:USTC_China_iGEM13_BBa_K1074006_1.png|thumb|left|800px|figure 1 SDS-PAGE of N-terminal TD1 modified GFP]]
+
[[Image:USTC_China_iGEM13_BBa_K1074006_1.png|thumb|left|400px|figure 1 SDS-PAGE of N-terminal TD1 modified GFP]]
−
[[Image:USTC_China_iGEM13_BBa_K1074006_4.png|thumb|left|800px|figure 2 SDS-PAGE of N-terminal TD1 modified LTB and HBsAg]]
+
[[Image:USTC_China_iGEM13_BBa_K1074006_4.png|thumb|right|400px|figure 2 SDS-PAGE of N-terminal TD1 modified LTB and HBsAg]]
 
[[Image:USTC_China_iGEM13_BBa_K1074006_2.JPG|thumb|left|600px|figure 3 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction]]
 
[[Image:USTC_China_iGEM13_BBa_K1074006_2.JPG|thumb|left|600px|figure 3 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction]]
 
[[Image:USTC_China_iGEM13_BBa_K1074006_5.jpg|thumb|left|800px|figure 4 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg ]]
 
[[Image:USTC_China_iGEM13_BBa_K1074006_5.jpg|thumb|left|800px|figure 4 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg ]]
  
 
|};
 
|};

Revision as of 13:11, 27 September 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1074006

User Reviews

UNIQ072f820f5c998e89-partinfo-00000000-QINU UNIQ072f820f5c998e89-partinfo-00000001-QINU

•••

USTC_China iGEM13

In our project, we construct other four gene circuits based on this part. In these gene circuits, GFP is replaced by HBsAg, Ag85b, PAD4, LTB respectively. We transformed the recombinant Plasmids both in BL21 and Bacillus Subtilis WB800N and induced expression with IPTG . Results analyzed by SDS-page , mass spectrum and ELISA were proved positive. But proteins expressed in WB800N were far less than those in BL21. The antigenicity and transdermal function of N-terminal TD1 modified antigen HBsAg was also tested positive by ELISA.

figure 1 SDS-PAGE of N-terminal TD1 modified GFP
figure 2 SDS-PAGE of N-terminal TD1 modified LTB and HBsAg
figure 3 Fluorescence detection of N-terminal TD1 modified GFP expression with and without IPTG induction
figure 4 ELISA test transdermal function of N-terminal TD1 modified antigen HBsAg
;