Difference between revisions of "Part:BBa K1036001"
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| − | <i>ndh</i> is the coding gene for NADH dehydrogenase II (<a href="http://www.ncbi.nlm.nih.gov/gene/8183397">NCBI</a>), a non-proton-translating single subunit membrane bond enzyme important in maintaining a redox-balanced dinucleotide pool. NADH dehydrogenase II is the preponderant respiratory source of H<sub>2</sub>O<sub>2</sub> as well as O<sub>2</sub><sup>-</sup>. In fact, substantial H<sub>2</sub>O<sub>2</sub> was formed when quinoneless vesicles containing only NADH dehydrogenase II were incubated with NADH.<br/>Earlier studies estimated that the formation rate of intracellular O<sub>2</sub><sup>-</sup> was about 10<sup>-5</sup> M S<sup>-1</sup> by extrapolation of O<sub>2</sub><sup>-</sup> yield from this enzyme. And NADH dehydrogenase II formed about 4 O<sub>2</sub><sup>-</sup> per 10 molecules of H<sub>2</sub>O<sub>2</sub> both during respiration and in the quinoneless membranes. | + | <i>ndh</i> is the coding gene for NADH dehydrogenase II (<a href="http://www.ncbi.nlm.nih.gov/gene/8183397">NCBI</a>), a non-proton-translating single subunit membrane bond enzyme important in maintaining a redox-balanced dinucleotide pool. NADH dehydrogenase II is the preponderant respiratory source of H<sub>2</sub>O<sub>2</sub> as well as O<sub>2</sub><sup>-</sup>. In fact, substantial H<sub>2</sub>O<sub>2</sub> was formed when quinoneless vesicles containing only NADH dehydrogenase II were incubated with NADH.<br/><br/> |
| + | Earlier studies estimated that the formation rate of intracellular O<sub>2</sub><sup>-</sup> was about 10<sup>-5</sup> M S<sup>-1</sup> by extrapolation of O<sub>2</sub><sup>-</sup> yield from this enzyme. And NADH dehydrogenase II formed about 4 O<sub>2</sub><sup>-</sup> per 10 molecules of H<sub>2</sub>O<sub>2</sub> both during respiration and in the quinoneless membranes.<br/><br/> | ||
| + | In our oscillatoral circuit, <i>ndh</i> just like a "conductor" because this gene could express NDH-2 which is a membrane-bound respiratory enzyme that produces low levels of H<sub>2</sub>O<sub>2</sub> and superoxide. H<sub>2</sub>O<sub>2</sub> will trigger synchronized oscillation between colonies. | ||
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Revision as of 12:56, 27 September 2013
the ndh gene coding for respiratory NADH dehydrogenase II
Description
ndh is the coding gene for NADH dehydrogenase II (NCBI), a non-proton-translating single subunit membrane bond enzyme important in maintaining a redox-balanced dinucleotide pool. NADH dehydrogenase II is the preponderant respiratory source of H2O2 as well as O2-. In fact, substantial H2O2 was formed when quinoneless vesicles containing only NADH dehydrogenase II were incubated with NADH.
Earlier studies estimated that the formation rate of intracellular O2- was about 10-5 M S-1 by extrapolation of O2- yield from this enzyme. And NADH dehydrogenase II formed about 4 O2- per 10 molecules of H2O2 both during respiration and in the quinoneless membranes.
In our oscillatoral circuit, ndh just like a "conductor" because this gene could express NDH-2 which is a membrane-bound respiratory enzyme that produces low levels of H2O2 and superoxide. H2O2 will trigger synchronized oscillation between colonies.
Experimental Data
Construciton
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1360
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]