Difference between revisions of "Part:BBa K1036004"
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Revision as of 12:36, 27 September 2013
sfgfp with LVA-tag
Description
This newly found GFP variant Superfolder GFP, which has shown outstanding performance in many ways. It has two advantages in observing on microfluidic: 1)Fold 3.5 to 4 times faster than regular GFP; 2)Yield up to four times more total Fluorescence than regular GFP. The greater the fluorescence strength is, the shorter exposure time will be needed, thus can decrease the photobleaching (GFP will be eventually destroyed by the light exposure that tries to stimulate it into fluorescing) that will lead into lapses in data processing.
More detailed characterization was performed by Cambridge iGEM team 2008(http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP).
Experimental Data
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 13