Difference between revisions of "Part:BBa K1228006"
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== Experiment and Results == | == Experiment and Results == | ||
<p>1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.</p> | <p>1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.</p> | ||
| − | < | + | <p>2. Add 200ul indicator inoculum (''Micrococcus Lysodeikticus'' or ''Escherichia Coli'') untill heated LB solid medium (100ml) cool to about 50°C.</p> |
| − | 3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight. | + | <p>3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight.</p> |
4. Observe whether LB plate will appear inhibition zone. | 4. Observe whether LB plate will appear inhibition zone. | ||
. | . | ||
Revision as of 11:04, 27 September 2013
25aa with T1 terminator and Constitutive promoter veg
This generator contains BBa_K541503(https://parts.igem.org/Part:BBa_K541503) and BBa_K1228005(https://parts.igem.org/Part:BBa_K1228005). The generator can secrete 25 aminoacid peptide that is a part of Lactoferrin in B.subtilis. It has a broad anti-bacterial range and strong anti-bacterial activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experiment and Results
1. Inoculate recombinant strains into 20ml LB.Cultivate at 37°C overnight.
2. Add 200ul indicator inoculum (Micrococcus Lysodeikticus or Escherichia Coli) untill heated LB solid medium (100ml) cool to about 50°C.
3. Use puncher make several hole on the LB plate.Add 80ul recombinant strains inoculum into the hole.Cultivate at 37°C overnight.
4. Observe whether LB plate will appear inhibition zone. .
Reference
1.BellamyW,TakaseM,Yamauchi K,et al. Identification of the bactericidal domainof lactoferrin[J]. BiochimicaetBiophysicaActa,1992,1121( 1/2):130-136. 2.EliassenLT,Berge G,SveinbjornssonB,et al. Evidenceforadirect antitumormechanismof actionof Bovinel actoferricin[J].AnticancerResearch,2002,22(5):2703－2710. 3.Wakabayashi H,TakaseM,TomitaM. Lactoferricin derived from milk protein lactoferrin[J]. Current Pharmaceutical Design,2003,9(16):1277－1287.