Difference between revisions of "Part:BBa K1065105:Design"
(→‎Design Notes)
 
Line 15: Line 15:
  
 
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT  
 
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT  
 +
 +
  
 
SAM synthetase was inserted into BBa_R0010 through ''standard assembly''.
 
SAM synthetase was inserted into BBa_R0010 through ''standard assembly''.

Latest revision as of 10:41, 27 September 2013

lac_promoter + RBS + SAM synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 904
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene sequence was extracted using the following primers:

>Primer Forward: prefix + RBS + spacer + ATG...

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT

>Primer Reverse: sequence_TAATAA_suffix

GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT


SAM synthetase was inserted into BBa_R0010 through standard assembly.

Source

SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.

References

  1. Ethylene biosynthesis and its regulation in higher plants
    Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89.
  2. MetaCyc EC 2.5.1.6, MetaCyc. August 15th, 2013, from link
  3. Ethylene biosynthesis and signaling networks
    Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151