Difference between revisions of "Part:BBa K1065105:Design"
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<partinfo>BBa_K1065105 short</partinfo> | <partinfo>BBa_K1065105 short</partinfo> | ||
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GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT | GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT | ||
| − | + | SAM synthetase was inserted into BBa_R0010 through ''standard assembly''. | |
===Source=== | ===Source=== | ||
Revision as of 10:41, 27 September 2013
lac_promoter + RBS + SAM synthetase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 324
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 904
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene sequence was extracted using the following primers:
>Primer Forward: prefix + RBS + spacer + ATG...
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
>Primer Reverse: sequence_TAATAA_suffix
GAATTCGCGGCCGCTTCTAGAGAAGGAGGAACTACTATGGCAAAACACCTTTTT
SAM synthetase was inserted into BBa_R0010 through standard assembly.
Source
SAM synthetase gene sequence extracted by PCR from E. coli MG1655 strain genome.
References
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Ethylene biosynthesis and its regulation in higher plants
Yang, S. F.; Hoffman N. E. Ann. Rev. Plant Physiol. 1965, 35, 155-89. - MetaCyc EC 2.5.1.6, MetaCyc. August 15th, 2013, from link
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Ethylene biosynthesis and signaling networks
Wang, K. L.-C.; Li, H.; Ecker J. R. Plant Cell 2002, Supplement, S131-S151