Difference between revisions of "Part:BBa K1228008"

(→‎Experiment)
(→‎Experiment)
Line 22: Line 22:
  
 
https://static.igem.org/mediawiki/2013/0/0a/L2_%E6%8A%91%E8%8F%8C%E5%9C%88_Mly.jpg
 
https://static.igem.org/mediawiki/2013/0/0a/L2_%E6%8A%91%E8%8F%8C%E5%9C%88_Mly.jpg
 +
 +
{{:Team：HZAU-China/background-head}}
 +
<html>
 +
<script type="text/javascript" src="/ajaxjs/jquery1.3.2.js"></script>
 +
<style type="text/css">
 +
#tip  {position:absolute;color:#333;display:none;z-index:1000;}
 +
#tip s  {position:absolute;top:40px;left:-20px;display:block;width:0px;height:0px;font-size:0px;line-height:0px;border-color:transparent #BBA transparent transparent;border-style:dashed solid dashed dashed;border-width:10px;z-index:1000;}
 +
#tip s i  {position:absolute;top:-10px;left:-8px;display:block;width:0px;height:0px;font-size:0px;line-height:0px;border-color:transparent #fff transparent transparent;border-style:dashed solid dashed dashed;border-width:10px;z-index:1000;}
 +
#tip .t_box  {position:relative;background-color:#CCC;filter:alpha(opacity=50);-moz-opacity:0.5;bottom:-3px;right:-3px;z-index:1000;}
 +
#tip .t_box div  {position:relative;background-color:#FFF;border:1px solid #ACA899;background:#FFF;padding:1px;top:-3px;left:-3px;z-index:1000;}
 +
 +
.tip  {width:82px;height:82px;}
 +
</style>
 +
<script type="text/javascript">
 +
$(function(){
 +
  $('.tip').mouseover(function(){
 +
      var $tip=$('<div id="tip"><div class="t_box"><div><s><i></i></s><img src="'+this.src+'" /></div></div></div>');
 +
      $('body').append($tip);
 +
      $('#tip').show('fast');
 +
  }).mouseout(function(){
 +
      $('#tip').remove();
 +
  }).mousemove(function(e){
 +
      $('#tip').css({"top":(e.pageY-60)+"px","left":(e.pageX+30)+"px"})
 +
  })
 +
})
 +
</script>
 +
 +
<style>
 +
#background{
 +
    background:url(https://static.igem.org/mediawiki/2013/8/80/Old_wall.jpg);
 +
    float:left;
 +
    min-heiht:600px;
 +
    width: 1000px;
 +
}
 +
#sidebar {
 +
    background-color:transparent;
 +
    clear: both;
 +
    float: left;
 +
    height: auto;
 +
    width: 240px;
 +
    padding-top: 10px;
 +
    padding-left: 15px;
 +
}
 +
#paragraphs {
 +
    background-color: transparent;
 +
    float: right;
 +
    height: auto;
 +
    text-align: justify;
 +
    padding-top: 10px;
 +
    padding-right:15px;
 +
    width: 710px;
 +
}
 +
</style>
 +
 +
<style>
 +
*{margin:0px;padding:0px;}
 +
ul.menu_scroll{z-index:100;padding:10px;margin:0 auto;padding-bottom: 5px;top:20px;position:fixed;width:100%;}
 +
ul.menu:after{content:"";display:block;height:0;clear:both;visibility:hidden;}
 +
ul.menu,
 +
ul.menu ul {padding:0;margin:0;list-style:none;position:;width:220px;background:#ddd;font-family:arial, sans-serif;}
 +
ul.menu {z-index:100;padding:10px;margin:0 auto;}
 +
ul.menu ul {z-index:50;
 +
-webkit-transition: 0.5s;
 +
-moz-transition: 0.5s;
 +
-ms-transition: 0.5s;
 +
-o-transition: 0.5s;
 +
transition: 0.5s;
 +
}
 +
ul.menu li {line-height:30px;position:relative;}
 +
ul.menu > li {margin-top:2px;font-size:12px;}
 +
ul.menu > li a {font:normal 16px/29px arial, sans-serif;color:#fff;text-decoration:none;}
 +
ul.menu label.open {display:block;background:#c00  no-repeat 170px 12px;line-height:30px;position:relative;z-
 +
 +
index:100;font:normal 12px/30px arial, sans-serif;color:#fff;border-radius:10px 10px 0 0;}
 +
ul.menu label.open b {color:#ff0;}
 +
ul.menu span {display:block;background:#c00;line-height:30px;position:relative;z-index:100;font-size: 16px;padding-left:10px;}
 +
ul.menu label img {position:absolute;left:0;top:0;width:100%;height:30px;}
 +
ul.menu ul li {margin-top:-30px;
 +
-webkit-transition: 0.5s;
 +
-moz-transition: 0.5s;
 +
-ms-transition: 0.5s;
 +
-o-transition: 0.5s;
 +
transition: 0.5s;
 +
}
 +
ul.menu ul li a {display:block;font:normal 16px/29px arial, sans-serif;color:#000;background:#ccc;}
 +
ul.menu ul li a:hover {background:#ddd;}
 +
ul.menu input {position:absolute;left:-9999px;}
 +
ul.menu li input:checked + label {background:#069;}
 +
ul.menu li input:checked ~ ul {background:#ccc;padding-bottom:10px;}
 +
ul.menu li input:checked ~ ul li {margin-top:0;}
 +
ul.menu label.close {display:block;width:200px;height:30px;background:transparent url("/jscss/demoimg/201208/u-arrow.gif")
 +
 +
no-repeat 170px 12px;line-height:30px;position:relative;left:0;z-index:90;margin-top:-30px}
 +
ul.menu input.tabs:checked ~ label.close {display:block;z-index:200;}
 +
ul.menu input.close:checked + label.close {display:none;}
 +
ul.menu input.close:checked ~ ul {background:#ddd;padding-bottom:0;}
 +
ul.menu input.close:checked ~ ul li {margin-top:-30px;}
 +
</style>
 +
 +
<script>
 +
$(document).ready(function(){
 +
var topMain=$("#sidebar").height()+100//是头部的高度加头部与nav导航之间的距离
 +
var menu=$(".menu");
 +
$(window).scroll(function(){
 +
if ($(window).scrollTop()>topMain){//如果滚动条顶部的距离大于topMain则就nav导航就添加类.nav_scroll，否则就移除
 +
menu.addClass("menu_scroll");
 +
}else{
 +
menu.removeClass("menu_scroll");
 +
}
 +
});
 +
})
 +
</script>
 +
 +
<body>
 +
<div id="background">
 +
    <div id="sidebar">
 +
        <body>
 +
        <ul class="menu">
 +
        <li><a href="http://2013.igem.org/Team:HZAU-China/Meeting Record"><span>Meeting Record</span></a></li>
 +
        <li><a href="http://2013.igem.org/Team:HZAU-China/Gallery"><span style="font-size:19px;color=#fff;">Gallery</span></a></li>
 +
        <li><a href="http://2013.igem.org/Team:HZAU-China/Protocol"><span>Protocol</span></a></li>
 +
      </body>
 +
    </div>
 +
    <div id="paragraphs">
 +
    <p><br></p>
 +
    <center><span style="font-size:46px;font-family:Cambria;margin-top:10px;line-height:80%">Gallery</span></center>
 +
    <p><br></p>
 +
   
 +
<p> </p>
 +
<a><img class="tip" src="https://static.igem.org/mediawiki/2013/7/7b/234.png" /></a>
 +
<a><img class="tip" src="https://static.igem.org/mediawiki/2013/7/7b/234.png" /></a>
 +
<a><img class="tip" src="https://static.igem.org/mediawiki/2013/7/7b/234.png" /></a>
 +
    </div>
 +
</div>
 +
</body>
 +
</html>
 +
 +
{{:Team：HZAU-China/background-footer}}
 +
<br/>
  
 
== Reference ==
 
== Reference ==

Revision as of 08:41, 27 September 2013

38aa with T1 terminator and Constitutive promoter veg

Human lysozyme (hLZ), the actual protein in breast milk,exhibits microbicidal activity to various degrees against several bacterial strains.The HLH peptide and its N-terminal helix (H1) were significantly the most potent bactericidal to Gram-positive and Gram-negative bacteria.Evidence shows that HLH peptide and its N-terminal helix (H1) kill bacteria by crossing the outer membrane of Gram-negative bacteria via self-promoted uptake and are able to dissipate the membrane potential-dependent respiration of Gram-positive bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Experiment

In order to distinguish whether the B. Subtilisexpress our objective protein or not,we used the method of in-vitro bacterial inhibition zone to observe the anti-bacterial effect.We added 100 ul B. Subtilis liquid medium into the hole. The inhibition zone results showed that gram-positive bacterium was sensitive to 38aa.

L2_%E6%8A%91%E8%8F%8C%E5%9C%88_Mly.jpg

Team：HZAU-China/background-head

Team：HZAU-China/background-footer

Reference