Difference between revisions of "Part:BBa K1073019"
| Line 6: | Line 6: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | In combination with a promoter of your choice this device can be used to create a RhlI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of | + | In combination with a promoter of your choice this device can be used to create a RhlI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of RhlI. |
The N-buturyl-HSL autoinducer molecules produced by RhlI bind to RhlR. This complex will bind to specific sequences in the regulatory region of the rhl promoter and induce gene expression. | The N-buturyl-HSL autoinducer molecules produced by RhlI bind to RhlR. This complex will bind to specific sequences in the regulatory region of the rhl promoter and induce gene expression. | ||
Revision as of 08:06, 27 September 2013
Autoinducer synthetase RhlI ( producing N-butyryl-HSL) with RBS and double terminator
The RhlI protein is a synthetase which produces the chemical autoinducer signal N-buturyl-HSL. In this device it is combined with a RBS and a double terminator in order to facilitate the construction of an expression cassette containing RhlI.
Usage and Biology
In combination with a promoter of your choice this device can be used to create a RhlI expression cassette. The RBS used will tune a medium expression level. Rate of transcription can be tuned by strength of promoter. The double terminator will prevent further transcription downstream of RhlI.
The N-buturyl-HSL autoinducer molecules produced by RhlI bind to RhlR. This complex will bind to specific sequences in the regulatory region of the rhl promoter and induce gene expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 680
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]