Difference between revisions of "Part:BBa K1189018"

Revision as of 08:00, 27 September 2013

Human ferritin di-subunit with E coil w/ LacI promoter

This part was created by fusing the heavy chain and light chains (PARtx2) of human ferritin together. It is expressed under the lacI promoter (PARt) and has a his-tag for protein purification. An E-coil (PARt) is included in order to allow binding of parts containing the respective K-coil (PART).

This construct can be used as a reporter through a modification of the iron core to form Prussian Blue.

Figure 4. Comparison image of commercial ferritin to Prussian blue ferritin after the synthesis reaction. The synthesis reaction took place over a 12 hour time period.

The resulting molecuel can then catalyse the formation of radicals from hydrogen perxoide, which can then cause a colour change in substrates such as TMB or ABTS.
https://static.igem.org/mediawiki/2013/c/c7/UCalgary2013TRSubstratecolourpartspage.png‎
<p> Figure 11. Image of the colours of ABTS and TMB (10 mg/mL for both) after reacting with Prussian blue ferritin.

Something about scaffold…

Sequence and Features