Difference between revisions of "Part:BBa K1139019"
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M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | M13 is a filamentous phage that infects only F+ strains of <i>E. coli</i>, which does not kill the host cell. <br> | ||
| − | This part is derived from M13mp18 phage vector, amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br> | + | This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of <i>g2p</i> is deleted. The capsid is formed only when <i>g2p</i> is expressed.<br> |
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Revision as of 07:47, 27 September 2013
Promoterless-M13 on pSB3
M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell.
This part is derived from M13mp18 phage vector(NEB), amplified by PCR. It includes 11 ORFs, M13 origin and a packaging sequence. The promoter on the upstream of g2p is deleted. The capsid is formed only when g2p is expressed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6027
- 1000COMPATIBLE WITH RFC[1000]