Difference between revisions of "Part:BBa K1073009"

Revision as of 07:31, 27 September 2013

LasI synthetase + LVA with subsequent double terminator

This construct is derived from BBa_C0078 and BBa_B0015. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL) including the LVA degradation tag with a double terminator to prevent further transciption.

Usage and Biology

The synthetase LasI produces the autoinducer molecule N-3-oxo-dodecanoyl-HSL of the Las quorum sensing system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 661
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 223
1000
COMPATIBLE WITH RFC[1000]

Revision as of 14:43, 26 September 2013 (view source) Laura S (Talk \| contribs) ← Older edit		Revision as of 07:31, 27 September 2013 (view source) Kerstin K (Talk \| contribs) Newer edit →
Line 2:		Line 2:
	<partinfo>BBa_K1073009 short</partinfo>		<partinfo>BBa_K1073009 short</partinfo>

−	This construct is derived from <partinfo>BBa_C0078</partinfo> and <partinfo>BBa_B0015</partinfo>. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL)including the LVA degradation tag with a double terminator to prevent further transciption.	+	This construct is derived from <partinfo>BBa_C0078</partinfo> and <partinfo>BBa_B0015</partinfo>. It combines the autoinducer synthase LasI (producing N-3-oxo-dodecanoyl-HSL) including the LVA degradation tag with a double terminator to prevent further transciption.