Difference between revisions of "Part:BBa K1036005:Design"

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===References===
 
===References===
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1. <a href="http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP">http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP</a><br/>
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2. <a href="http://www.ncbi.nlm.nih.gov/pubmed/16369541">Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)</a><br/>
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3. <a href="http://www.ncbi.nlm.nih.gov/pubmed?term=A%20sensing%20array%20of%20radically%20coupled%20genetic%20'biopixels'.[all]&cmd=correctspelling">Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)</a>
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Revision as of 07:31, 27 September 2013


sfgfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 914
    Illegal BsaI.rc site found at 2017
    Illegal SapI.rc site found at 31


Design Notes

it is used to construct sfGFP


Source

The template of sfgfp gene namely biobrick pSB1C3-RBS-sfgfp-TT was provided by 2013 iGEM team Peking.We add the LVA-tag in the end of sfgfp.
We thanks for Peking's help.

References

1. http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP
2. Pedelacq J., Cabantous S., Tran T., Terwilliger T. & Waldo G. S. Engineering and characterization of a superfolder green fluorescent protein. Nature 24, 79-88 (2006)
3. Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)