Difference between revisions of "Part:BBa K1036002:Design"

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We used the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_F2621">BBa_F2621</a> (located in 2012-Plate 2-21F)
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We used the biobrick <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D).
 
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Latest revision as of 07:02, 27 September 2013


gfp (with LVA-tag) under plux R control


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2743
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 662
    Illegal BsaI.rc site found at 920
    Illegal BsaI.rc site found at 2023


Design Notes

This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D). BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.

Source

We used the biobrick BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D).

References

Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)