Difference between revisions of "Part:BBa K364202"
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<partinfo>BBa_K364202 parameters</partinfo>
 
<partinfo>BBa_K364202 parameters</partinfo>
 
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==Contribution: iGEM2013_Team SYSU-China==
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Group:SYSU-China 2013
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Author:Sun Meng Yi
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We express this part in yeast, observe the growth inhibition effect, and compare it with its mutant form. We also submit the mutant form to registry, and the data and link is here:https://parts.igem.org/wiki/index.php?title=Part:BBa_K1061006
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Revision as of 07:01, 27 September 2013

hBAX

Coding sequence of Bax (Bcl-2-associated X) protein which is a pro-apoptotic member of the Bcl-2 protein family. It contains three BH (Bcl-2 homology) domains, can form homo- and heterodimers and plays essential role in the formation of MOMP (Mitochondrial outer membrane permeabilization) leading to apoptosis through cytochrome c release which is the most frequent type of PCD (Programmed cell death).

Picture of gel electrophoresis: hBAX in pSB1C3 resulting an insert of 573 bp. Circular pSB1C3-hBAX and restricted PMAT-hBAX can be seen as control on the gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 449
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Contribution: iGEM2013_Team SYSU-China

Group:SYSU-China 2013 Author:Sun Meng Yi We express this part in yeast, observe the growth inhibition effect, and compare it with its mutant form. We also submit the mutant form to registry, and the data and link is here:https://parts.igem.org/wiki/index.php?title=Part:BBa_K1061006