Difference between revisions of "Part:BBa K1036002:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| − | + | This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> digested by <i>Eco</i>R & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R & <i>Xba</i>I were ligated together by T4 ligase. | |
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===Source=== | ===Source=== | ||
Revision as of 06:40, 27 September 2013
gfp (with LVA-tag) under plux R control
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2743
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 662
Illegal BsaI.rc site found at 920
Illegal BsaI.rc site found at 2023
Design Notes
This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D). BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.
Source
iGEM
References
Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)