Difference between revisions of "Part:BBa K1139100"

Revision as of 06:39, 27 September 2013

Adenylate isopentenyltransferase 4

In order to construct cytokinin production part, we focused on AtIPT, a plant enzyme which catalyzes the synthesis of cytokinin. The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001). We ordered a DNA sequence of AtIPT (AtIPT4) derived from A. thaliana and are now constructing the part including one of them.

Fig. 1. Mechanisms of cytokinin synthesis

At<i>IPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli. It is also expected in the report that E. coli also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP.

For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Project/Farming our work in Tokyo_Tech 2013 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 328
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 61
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1139100 short</partinfo>
-In order to construct cytokinin production part, we focused on AtIPT, a plant enzyme which catalyzes the synthesis of cytokinin.  The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001).  We ordered a DNA sequence of AtIPT (AtIPT4) derived from A. thaliana and are now constructing the part including one of them.<br>
+In order to construct cytokinin production part, we focused on <i>At</i>IPT, a plant enzyme which catalyzes the synthesis of cytokinin.  The mechanisms of cytokinin synthesis are shown in Fig. 1 (Takei et al., 2001).  We ordered a DNA sequence of <i>At</i>IPT (<i>At</i>IPT4) derived from <i>A. thaliana</i> and are now constructing the part including one of them.<br>
 [[Image:titech2013_parts_K1139100_main_Fig1.jpg|thumb|center|500px|<b>Fig. 1.</b> Mechanisms of cytokinin synthesis]]
-AtIPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli</i>.  It is also expected in the report that <i>E. coli</i> also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP. <br>
+<i>At<i>IPT, a plant enzyme, catalyzes the synthesis of iPRMP from DMAPP and AMP. According to the previous research, DMAPP, and AMP are provided by authentic metabolism in <i>E. coli</i>.  It is also expected in the report that <i>E. coli</i> also has the enzymes which catalyze the synthesis of iP or tZ from iPRMP. <br>
 For more information, see [http://2013.igem.org/Team:Tokyo_Tech/Project/Farming our work in Tokyo_Tech 2013 wiki].