Difference between revisions of "Part:BBa K1036003:Design"
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| − | This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D).<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> digested by <i>Eco</i>R & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R & <i>Xba</i>I were ligated together by T4 ligase. | + | This part contains two subparts: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> (located in 2013-Plate 3-11H) and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> (located in 2013-Plate 1-12D). <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_I763020">BBa_I763020</a> digested by <i>Eco</i>R & <i>Spe</i>I and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K546000">BBa_K546000</a> digested by <i>Eco</i>R & <i>Xba</i>I were ligated together by T4 ligase. |
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Revision as of 06:38, 27 September 2013
lux pL controlled luxR with lux pR controlled gfp (LVA-tag)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3909
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1101
Illegal BsaI.rc site found at 1828
Illegal BsaI.rc site found at 2086
Illegal BsaI.rc site found at 3189
Design Notes
This part contains two subparts: BBa_I763020 (located in 2013-Plate 3-11H) and BBa_K546000 (located in 2013-Plate 1-12D). BBa_I763020 digested by EcoR & SpeI and BBa_K546000 digested by EcoR & XbaI were ligated together by T4 ligase.
Source
iGEM
References
Prindle, A., Samayoa, P., Razinkov, I., Danino, T., Tsimring, L.S., & Hasty, J. A sensing array of radically coupled genetic 'biopixels'. Nature 481, 39−44. (2012)