Difference between revisions of "Part:BBa K1189004:Design"
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===Design Notes=== | ===Design Notes=== | ||
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We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. | We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone. | ||
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===Source=== | ===Source=== | ||
Revision as of 03:59, 27 September 2013
TALEA Target ([A])
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 213
Illegal AgeI site found at 325 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed primers that would produce these TALE A target sequence in a KAPA PCR reaction. Our target sequences were then ligated into RFP generator, in a pSB1C3 backbone.
Source
Primers