Difference between revisions of "Part:BBa J100129:Experinece"
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| − | Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into [[Part:BBa_J119137]], and it we had, the colonies would not be green. Unfortunately, all of ours were green. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. | + | Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into [[Part:BBa_J119137]], and it we had, the colonies would not be green. Unfortunately, all of ours were green. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is [[Part:BBa_J04450]] with a strong promoter. |
[[File:113SP.png]] | [[File:113SP.png]] | ||
Latest revision as of 20:17, 26 September 2013
Our promoter J100129 was not cloned successfully as indicated by the presence of just green fluorescence. We tried to clone it into Part:BBa_J119137, and it we had, the colonies would not be green. Unfortunately, all of ours were green. The following is a graph detailing the fluorescence per optical density. This is not a measure of our promoter since we did not clone it into the plasmid. This is part of an undergraduate biology lab assignment. Our positive control is Part:BBa_J04450 with a strong promoter.
