Difference between revisions of "Part:BBa K1074007"
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<partinfo>BBa_K1074007 short</partinfo> | <partinfo>BBa_K1074007 short</partinfo> | ||
− | Promoter ctc is a sigma factor B-dependent promoter | + | Promoter ctc(<partinfo>BBa_K143010</partinfo>) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress. amilCP(<partinfo>BBa_K592009</partinfo>), blue chromoprotein,naturally exhibits strong color when expressed. We assemble this gene circuit to expect a visual signal when B.subtilis grow to the post-log phase. |
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Revision as of 19:04, 26 September 2013
Pctc+RBS+amilCP
Promoter ctc(BBa_K143010) is a sigma factor B-dependent promoter in B. subtilis. Activated by endogenous sigma factor B under mild stress. amilCP(BBa_K592009), blue chromoprotein,naturally exhibits strong color when expressed. We assemble this gene circuit to expect a visual signal when B.subtilis grow to the post-log phase.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 78
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 78
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 78
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 78
- 1000COMPATIBLE WITH RFC[1000]