Difference between revisions of "Part:BBa C0083:Design"

Revision as of 19:37, 21 July 2006

aspartate ammonia-lyase

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 72
Illegal BglII site found at 1537
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 964
Illegal AgeI site found at 493
Illegal AgeI site found at 1345
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1004

Design Notes

The start codon was changed from GTG to ATG to match BioBricks standards.

Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.

Source

Database Entries:

UniProt #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=ASPA_ECOLI P0AC38;P04422;P78140;Q2M6G5]
Entrez Gene #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=948658 948658]

Source Organism: E. coli K12

References

Budrene, E. O. and Berg, H. C. Dynamics of formation of symmetrical patterns by chemotactic bacteria. Nature 376, 49-53 (1995).

@@ Line 17: / Line 17: @@
 Database Entries:
 *UniProt #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=ASPA_ECOLI P0AC38;P04422;P78140;Q2M6G5]
+*Entrez Gene #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=948658 948658]
 *Source Organism: E. coli K12