Difference between revisions of "Part:BBa K1061001"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1061001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1061001 SequenceAndFeatures</partinfo>
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====Calibration====
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====calibration====
 
Pre-experiment that we done
 
Pre-experiment that we done
 
[[Image:Suicidegene-vp3.png|thumb|center|500px|apoptosis effect ]]
 
[[Image:Suicidegene-vp3.png|thumb|center|500px|apoptosis effect ]]
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technique to confirm the apoptosis effect.Detail will be show in our wiki.http://2013.igem.org/Team:SYSU-China/Project/Results
 
technique to confirm the apoptosis effect.Detail will be show in our wiki.http://2013.igem.org/Team:SYSU-China/Project/Results
  
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====Kill range====
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For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this characteristic as important characteristic of suicide gene.
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===

Revision as of 17:05, 26 September 2013

apoptin

Apoptin is a protein that is originally isolated from the chicken anemia virus(CAV). It has been found that expressing this gene can induce apoptosis in a broad spectrum of cancer cells but not in normal cells. Hence it is a very powerful tool in treating cancer. Fusion the TAT protein with apoptin can even achieve by-stander effect, and we try to use it in the most simple device of our project. We have successfully expressed it in Hep G2 cell lines and observe a brilliant apoptosis effect.[1]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

calibration

Pre-experiment that we done

apoptosis effect

(Apoptosis phenotype of Apoptin(VP3). The protein was expressed in fusion with a GFP. Compared with 0 hr , apoptosis rose 48 hr after transfection, and GFP vision indicated the transfection efficiency)However, due to technical problem in cell culture,the transfection effciency can not reach such a high level in following experiment.The phenomena become not so obvious,but still exist.To observe that, we must carefully count the cells. So, finally we decided to use the Flow cytometry technique to confirm the apoptosis effect.Detail will be show in our wiki.http://2013.igem.org/Team:SYSU-China/Project/Results

Kill range

For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this characteristic as important characteristic of suicide gene.

References:

[1] Claude Backendorf et al,Apoptin: Therapeutic Potential of an Early Sensor of Carcinogenic Transformation,Annu. Rev. Pharmacol. Toxicol. 2008. 48:143–69