Difference between revisions of "Part:BBa K1017811"

Revision as of 16:36, 26 September 2013

Pcons+rRBS-2+mRFP+J61048

Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter. We made this biobrick in order to test whether our self-designed rRBS-2 really works. Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034.)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 784
Illegal AgeI site found at 625
Illegal AgeI site found at 737
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1017811 short</partinfo>
-Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter.And J61048 is a terminator.
+Pcons is a constitutive promoter which can be used to turn the expression level. rRBS-2 is a sRNA binding site that is designed by iGEM13_NCTU_Formosa. When the sRNA from K1017402 bind on it, this part will be shut down. Then, the downstream gene won't be made. mRFP(E1010) is a red fluorescent protein that functions as a reporter.
 We made this biobrick in order to test whether our self-designed rRBS-2 really works.
 Also, we want to compare this rRBS-2’s strength with other RBS(B0030, B0032, B0034.)