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<p>For detailed information concerning PaaX, please visit <a href="http://2013.igem.org/Team:Peking/Project/BioSensors/HcaR#Mileston3">2013 Peking iGEM Biosensor PaaX</a></p>
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Revision as of 16:35, 26 September 2013

J23114-PaaX

For detailed information concerning PaaX, please visit 2013 Peking iGEM Biosensor PaaX


Construction

In order to fine-tune the performance of PaaX biosensor, we constructed a library of Pc constitutive promoters at different intensity. BBa_K1031744 is composed of three elements, the constitutive Pc promoter J23114[1], coding sequence of PaaX and terminator B0015[2]. (Fig 1)

Fig 1 Construction of biosensor circuit Pa/J23114-PaaX. The orange arrowheads represent promoters. RBS are shown in green ovals. The square in dark red stands for terminator B0015.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]