Difference between revisions of "Part:BBa K1100062"

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[[File:Repression rate.jpg|600px|thumb|center|Figure1. Repression rate]]
 
[[File:Repression rate.jpg|600px|thumb|center|Figure1. Repression rate]]
 
[[File:S04.jpg|400px|thumb|center|Figure2. RNAIN/OUTS04 binding duplex]]
 
[[File:S04.jpg|400px|thumb|center|Figure2. RNAIN/OUTS04 binding duplex]]
 +
[[File:OUTS4.jpg|400px|thumb|center|Figure3. predicted structure of RNAOUTS4]]
  
 
References:
 
References:

Revision as of 16:08, 26 September 2013

RNA-OUT S4

It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:
Figure1. Repression rate
Figure2. RNAIN/OUTS04 binding duplex
Figure3. predicted structure of RNAOUTS4

References:

Mutalik V K, Qi L, Guimaraes J C, et al. Rationally designed families of orthogonal RNA regulators of translation[J]. Nature Chemical Biology, 2012, 8(5): 447-454.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]