Difference between revisions of "Part:BBa K1139019:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1139019 short</partinfo> | <partinfo>BBa_K1139019 short</partinfo> | ||
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TBD | TBD | ||
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| + | Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3' | ||
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| + | Rev primer:5'-CTGCAGCGGCCGCTACTAGTATTTCACCAGCGAGACGG-3' | ||
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===References=== | ===References=== | ||
Revision as of 15:53, 26 September 2013
Promoterless-M13 on pSB3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 99
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6027
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This biobrick is derived from M13mp18 phage vector, amplified by using PCR.
Source
TBD
Fwd primer:5'-ACGTGAATTCGCGGCCGCTTCTAGAGAACCGGGGTACATATGATTGAC-3'
Rev primer:5'-CTGCAGCGGCCGCTACTAGTATTTCACCAGCGAGACGG-3'