Difference between revisions of "Part:BBa K1100063"

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	<p></p>It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:		<p></p>It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:
	[[File:Repression rate.jpg|600px|thumb|center|Figure1.Repression rate]]		[[File:Repression rate.jpg|600px|thumb|center|Figure1.Repression rate]]
−	[[File:S05.jpg|400px|thumb|center|Figure2.RNAIN/OUTS04 binding duplex]]	+	[[File:S05.jpg|400px|thumb|center|Figure2.RNAIN/OUTS05 binding duplex]]
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Revision as of 15:52, 26 September 2013

RNA-OUT S5

It is amongst the mutant library of RNA-OUT, part of a well-known antisense RNA-mediated translation control system in E. coli called RNA-IN-RNA-OUT. It derived from the copy-number control element from the insertion sequence IS10, wherein the RNA-OUT inhibits transposase expression. RNA-OUT base pairs to the translation initiation region of the transposase mRNA (RNA-IN), thereby repressing translation both by preventing ribosome binding and by promoting transcription degradation. The 5’ end of the unstructured, unstable sense RNA-IN is complementary to the top of the loop domain and to one entire side of the stable RNA-OUT hairpin. Earlier studies have suggested that the 5 base pairs in the 5’ end of RNA-IN and in the loop domain of RNA-OUT determine the initiation of RNA duplex formation. The mutation of these 5 base pairs resulted in a library of orthogonal RNA regulators of translation. The translation repression rate of these regulators and the cross-talk of them are shown as followed:

Sequence and Features