Difference between revisions of "Part:BBa K1017101"

Revision as of 15:39, 26 September 2013

Pred ( PompC + B0032 + LacI + J61048 + Plac)

In general, the red light sensing promoter, OmpC promoter, turns on in the dark and turns off under red light with the present of Cph8.

But we want to construct a more intuitive light regulating th system at can turn on under red light and turn off in the dark. So we created a new biobrick named red promoter by adding a lac operon to inverse the function of OmpC promoter.

Pred, including PompC,RBS(B0032), LacI, ter(J61048), and Plac, can be activated when exposed to red light. OmpR, the transcription factor of PompC, is active when being phosphorylated in dark and inactive under red light. In the presence of red light, the non phosphorylated OmpR cannot turn on PompC. Thus, Plac won't be repressed by the negative regulator, LacI. So the whole Pred can express genes downstream Plac. On the other hand, if there is no red light, the phosphorylated OmpR then turn on PompC, causing LacI to be expressed and repress Plac to translate the genes downstream.

Sequence and Features