Difference between revisions of "Part:BBa C0061"

Revision as of 21:59, 20 July 2006

autoinducer synthetase for AHL

Synthesizes 3OC₆HSL, which binds to LuxR.

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Usage and Biology

Enzyme for creating acyl-homoserine lactones from normal cell metabolites. All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity. Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 637
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_C0061 short</partinfo>
-Synthesizes 3OC<sub>6</sub>HSL, which binds to LuxR.
+Synthesizes [[30C6HSL|3OC<sub>6</sub>HSL]], which binds to LuxR.
-The lux cassette of V. fischeri contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules      of LuxR protein form a complex with two molecules the signalling compound      HSL. This complex binds to a palindromic site on the promoter, increasing      the rate of transcription.
+The lux cassette of V. fischeri contains a left and a right promoter. The      right promoter gives weak constitutive expression of downstream genes.This      expression is up-regulated by the action of the [[Lux]] repressor, LuxR. Two molecules      of LuxR protein form a complex with two molecules the signalling compound     [[AHL|HSL]]. This complex binds to a palindromic site on the promoter, increasing      the rate of transcription.
@@ Line 11: / Line 10: @@
 Enzyme for creating acyl-homoserine lactones from normal cell metabolites.
+All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_C0061 SequenceAndFeatures</partinfo>