Difference between revisions of "Part:BBa K1119006:Design"
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===References===
 
===References===
−
pEGFP-N1 (Clonetech)
+
Clonetech.(1999)).pEGFP-N1 Vector Information.Retrieved from
−
http://www.addgene.org/vector-database/2491/
+
http://www.pkclab.org/PKC/vector/pEGFPN1.pdf

Revision as of 15:10, 26 September 2013

CMV promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Caution: If this promoter is fused to a mammalian translation unit using RFC10, the 5'UTR would only have 6nt. If users encounter lower or no expression upon assembly, including extra DNA spacer sequences between the CMV promoter and the first ATG codon might help.


Source

The CMV promoter sequence was cloned out from pEGFP-N1(Clonetech) using PCR with primers that includes prefix and suffix in RFC10 standard.

References

Clonetech.(1999)).pEGFP-N1 Vector Information.Retrieved from http://www.pkclab.org/PKC/vector/pEGFPN1.pdf