Difference between revisions of "Part:BBa K1017726"
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We design this part to change heme into phycocyanobilin(PCB). The following is the pathway.
 
We design this part to change heme into phycocyanobilin(PCB). The following is the pathway.
 +
The part of the photoreceptor that responds to light, phycocyanobilin, is not naturally produced in E. coli. We therefore introduced two phycocyanobilin-biosynthesis genes (ho1 and pcyA) from Synechocystis that convert haem into phycocyanobilin.
  
 
https://static.igem.org/mediawiki/2013/9/9b/Nctu_formosa_PCBsynthe.jpg
 
https://static.igem.org/mediawiki/2013/9/9b/Nctu_formosa_PCBsynthe.jpg
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In this part, Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part. And what we choose is J23101. B0030 and B0032 are strong ribosome binding sites(RBS), but they have different strength. pcyA and hol1 are two requisite genes which are required for the biosynthesis to change heme into PCB. hol1 will oxidizes the heme group then generate biliverdin IXalpha, and pcyA converts biliverdin IXalpha into phycocyanobilin(PCB).
 
In this part, Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part. And what we choose is J23101. B0030 and B0032 are strong ribosome binding sites(RBS), but they have different strength. pcyA and hol1 are two requisite genes which are required for the biosynthesis to change heme into PCB. hol1 will oxidizes the heme group then generate biliverdin IXalpha, and pcyA converts biliverdin IXalpha into phycocyanobilin(PCB).
  
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PCB is essential for Cph8 (BBa_K1017301) to absorb red light . It well combine to Cph1 as a chromophore .
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:41, 26 September 2013

Pcons+B0030+pcya+B0032+ho1+B0030


We design this part to change heme into phycocyanobilin(PCB). The following is the pathway. The part of the photoreceptor that responds to light, phycocyanobilin, is not naturally produced in E. coli. We therefore introduced two phycocyanobilin-biosynthesis genes (ho1 and pcyA) from Synechocystis that convert haem into phycocyanobilin.

Nctu_formosa_PCBsynthe.jpg


In this part, Pcons is a constitutive promoter family member(from J23100 to J23119) which can be used to tune the expression level of express part. And what we choose is J23101. B0030 and B0032 are strong ribosome binding sites(RBS), but they have different strength. pcyA and hol1 are two requisite genes which are required for the biosynthesis to change heme into PCB. hol1 will oxidizes the heme group then generate biliverdin IXalpha, and pcyA converts biliverdin IXalpha into phycocyanobilin(PCB).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 833
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
  • 1000
    COMPATIBLE WITH RFC[1000]