Difference between revisions of "Part:BBa K1119008:Design"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1119008 short</partinfo>
 
<partinfo>BBa_K1119008 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Later
+
There are NgoMIV & AgeI sites around GFP reporter ([[Part:BBa_K648013|BBa_K648013]]), which make the part incompatible with RFC25 standard.
−
 
+
  
  
 
===Source===
 
===Source===
−
 
+
HKUST iGEM team 2013
−
Later
+
  
 
===References===
 
===References===

Revision as of 14:37, 26 September 2013

CMV promoter - GFP - hGH polyA tail


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 614
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 628
    Illegal AgeI site found at 1369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1772
    Illegal BsaI.rc site found at 1274


Design Notes

There are NgoMIV & AgeI sites around GFP reporter (BBa_K648013), which make the part incompatible with RFC25 standard.


Source

HKUST iGEM team 2013

References