Difference between revisions of "Part:BBa K1084124:Design"
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===Source===
 
===Source===
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Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.
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coming soon
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===References===
 
===References===
−
Translation initiation region sequence preferences in Escherichia coli Vladimir Vimberg, Age Tats, Maido Remm and Tanel Tenson
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V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology

Revision as of 14:05, 26 September 2013

pTet SD8 LacZα dT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This RBS has an sequence called an "enhancer". The enhancer is located upstream of the SD seqeuence. It enhances the binding of Ribosome and mRNA. Also, it is said that the strength of the RBS, depends on the length of SD sequence.


Source

Based on Vimberg et al. (2007), we construnted this part from synthetic oligos.

References

V. Vimberg, A. Tats, M. Remm T. Tenson, Translation initiation region sequence preferences in Escherichia coli (2007) BMC Molecular Biology