Difference between revisions of "Part:BBa K1017404"
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the sRNA from effecting undesired genes, so it is necessarily to design | the sRNA from effecting undesired genes, so it is necessarily to design | ||
artificial sRNA that targets specifically to the desired genes. | artificial sRNA that targets specifically to the desired genes. | ||
| − | + | [[File:Nctu_formosa_sRNA_inhibition.JPG|500px|center]] | |
===How we design=== | ===How we design=== | ||
We first picked the sRNA from a library of artificial sRNA that was constructed by fusing a randomized antisense domain of Spot42, the scaffold that is known to recruit the RNA chaperons. As we want the sRNA we picked can specifically complementary to the SD sequence which in our designed RBS(BBa_K1017202[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1017202]), we picked the one which contains a consensus sequence, 5’-CCCUC-3’. Our artificial sRNA also has three stem-loop double stranded RNA structures, and the loop closest to the 3’ terminus is complementary to a sequence preceding the initiation codon of mRNA, so that it can prevent the ribosome from binding to the initiation condon, so the translation would be repressed. | We first picked the sRNA from a library of artificial sRNA that was constructed by fusing a randomized antisense domain of Spot42, the scaffold that is known to recruit the RNA chaperons. As we want the sRNA we picked can specifically complementary to the SD sequence which in our designed RBS(BBa_K1017202[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1017202]), we picked the one which contains a consensus sequence, 5’-CCCUC-3’. Our artificial sRNA also has three stem-loop double stranded RNA structures, and the loop closest to the 3’ terminus is complementary to a sequence preceding the initiation codon of mRNA, so that it can prevent the ribosome from binding to the initiation condon, so the translation would be repressed. | ||
Revision as of 13:36, 26 September 2013
sRNA-2
Description of function
Small RNA is an important role on gene regulation. It will interact with the targeted mRNA by imperfect basepairing, reducing the translational efficiency and recruiting chaperones such as Hfq for translational termination.Since we choose sRNA to be part of our regulated-system, we need to prevent the sRNA from effecting undesired genes, so it is necessarily to design artificial sRNA that targets specifically to the desired genes.
How we design
We first picked the sRNA from a library of artificial sRNA that was constructed by fusing a randomized antisense domain of Spot42, the scaffold that is known to recruit the RNA chaperons. As we want the sRNA we picked can specifically complementary to the SD sequence which in our designed RBS(BBa_K1017202[1]), we picked the one which contains a consensus sequence, 5’-CCCUC-3’. Our artificial sRNA also has three stem-loop double stranded RNA structures, and the loop closest to the 3’ terminus is complementary to a sequence preceding the initiation codon of mRNA, so that it can prevent the ribosome from binding to the initiation condon, so the translation would be repressed.
Quantitative data showing the Part or Device function
Acknowedgment of sources and references
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 3
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]