Difference between revisions of "Part:BBa K1036000"

(Description)
(Description)
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=Description=
 
=Description=
This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: [[BBa_K1036001]], here is a detailed description of quorum sensing promoter. Firstly, we should be familiar with the quorum sensing mechanism, the ''luxI'' gene is at low expression level and produces LuxI protein that synthesizes a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by ''luxR'' gene. The LuxR-AHL complex can activate the ''luxI'' promoter, and the positive feedback loop is built. However, since in this circuit the ''luxI'' gene behind the ''luxI'' promoter is changed into ''ndh'' gene (with LVA-tag), so it could only be activated by AHL produced by ''luxI'' gene on another plasmid, which contains Part: BBa_K1036003.
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[[BBa_K1036001]][[https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036001]]
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This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1036001">BBa_K1036001</a>, here is a detailed description of quorum sensing promoter. Firstly, we should be familiar with the quorum sensing mechanism, the ''luxI'' gene is at low expression level and produces LuxI protein that synthesizes a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by ''luxR'' gene. The LuxR-AHL complex can activate the ''luxI'' promoter, and the positive feedback loop is built. However, since in this circuit the ''luxI'' gene behind the ''luxI'' promoter is changed into ''ndh'' gene (with LVA-tag), so it could only be activated by AHL produced by ''luxI'' gene on another plasmid, which contains Part: BBa_K1036003.
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=Usage and Biology=
 
=Usage and Biology=

Revision as of 12:18, 26 September 2013

lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

Description

This part is made up of a quorum sensing promoter and ''ndh'' gene with a LAV-tag. Under control of the quorum sensing promoter, ''ndh'' gene will express periodically as the promoter is periodically activated and repressed. Since the ''ndh'' gene is described detailed in Part: BBa_K1036001, here is a detailed description of quorum sensing promoter. Firstly, we should be familiar with the quorum sensing mechanism, the ''luxI'' gene is at low expression level and produces LuxI protein that synthesizes a kind of acyl-homoserine lactone (AHL), which is a small molecule that can diffuse across the cell membrane and mediate intercellular coupling when it reaches the threshold as enough biomass accumulated. AHL will bind intracellular protein LuxR, which is also consecutively produced by ''luxR'' gene. The LuxR-AHL complex can activate the ''luxI'' promoter, and the positive feedback loop is built. However, since in this circuit the ''luxI'' gene behind the ''luxI'' promoter is changed into ''ndh'' gene (with LVA-tag), so it could only be activated by AHL produced by ''luxI'' gene on another plasmid, which contains Part: BBa_K1036003.

Usage and Biology

Protocol

Ndh with LVA tag is formed as a composite by the ligation of quorum sensing promoter luxR via digestion and ligation.

Experimental Data

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2544
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1101