Difference between revisions of "Part:BBa K1088012"

Revision as of 08:56, 26 September 2013

E. coli dxs (lac promoter without lac inhibitor)

This part has the dxs gene derived from E. coli (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.

Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs derived from B. subtilis. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 946

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 <partinfo>BBa_K1088012 short</partinfo>
-This part the ''dxs'' derived from ''E.coli'' (BBa_K1088000) controlled by the common lac promoter. Notice that overexpression of LacI is required for regulation of the Lac promoter on high copy plasmids.
+This part has the ''dxs'' gene derived from ''E. coli'' (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.
+Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs derived from ''B. subtilis''. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation).
 <!-- Add more about the biology of this part here