Difference between revisions of "Part:BBa K1045016:Design"
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The inverse DarR sequence was cut out from part BBa_K1045001 and ligated into part BBa_K1045009 in a post-fixing composition.
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The inverse DarR sequence was cut out from [[Part:BBa_K1045001|BBa_K1045001]] and ligated into [[Part:BBa_K1045009|BBa_K1045009]] in a post-fixing composition.
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===Source===
 
===Source===

Revision as of 08:47, 26 September 2013

Terminator BBa_B0015 with inversed Pre- and Suffix- DarR ORF with inversed Pre- and Suffix


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 731
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 168
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The inverse DarR sequence was cut out from BBa_K1045001 and ligated into BBa_K1045009 in a post-fixing composition.

Source

To be continued

References

Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096