Difference between revisions of "Part:BBa K1045015:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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The inverse RBS was generated by hybridizing oligos to a double-stranded DNA fragment that corresponded to the inverse RBS sequence cut with EcoRI and SpeI at the prefix and suffix sequence. Next, this hybridization product was cloned in a prefixing composition into [[Part:BBa_K1045014|BBa_K1045014]].
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The inverse RBS was generated by hybridizing oligos to a double-stranded DNA fragment that corresponded to the inverse RBS sequence cut with ''EcoRI'' and ''SpeI'' at the prefix and suffix sequence. Next, this hybridization product was cloned in a prefixing composition into [[Part:BBa_K1045014|BBa_K1045014]].
  
 
===Source===
 
===Source===

Revision as of 08:43, 26 September 2013

RBS BBa_B0034 with inversed Pre- and Suffix- Promoter reverse - Promoter - DarR operator - BBa_E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 21
    Illegal NheI site found at 44
    Illegal NheI site found at 124
    Illegal NheI site found at 147
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 845


Design Notes

The inverse RBS was generated by hybridizing oligos to a double-stranded DNA fragment that corresponded to the inverse RBS sequence cut with EcoRI and SpeI at the prefix and suffix sequence. Next, this hybridization product was cloned in a prefixing composition into BBa_K1045014.

Source

To be continued

References