Difference between revisions of "Part:BBa K1151000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | It was inserted in a pET-15b plasmid vector | + | It was inserted in a pET-15b plasmid vector. |
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===Source=== | ===Source=== | ||
Revision as of 07:39, 26 September 2013
Nickel-responsive pleiotropic regulator (HpNikR)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It was inserted in a pET-15b plasmid vector.
Source
It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.
References
1. Muller et al, Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75. 2. Dian et al, Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States. J Mol Biol. 2006; 25;361(4):715-30. 3. Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30. 4. Ernst et al, The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori . Infect Immun. 2005; 73(11):7252-8.