Difference between revisions of "Part:BBa K1014000:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
−
TAATAA double stop codon added.
+
<p></p>TAATAA double stop codon added.
−
Codon optimization was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).
+
<p></p>Codon optimization was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).
−
Results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]
+
<p></p>Results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]
  
 
===Source===
 
===Source===

Revision as of 05:30, 26 September 2013

HrpS Gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 157
    Illegal AgeI site found at 163
    Illegal AgeI site found at 298
    Illegal AgeI site found at 456
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TAATAA double stop codon added.

Codon optimization was done using [http://www.jcat.de/ JCat] an online Codon Adaptation Tool which was published in NAR (Nucleic Acids Research).

Results was present on [http://2013.igem.org/Team:HIT-Harbin/Parts our wiki]

Source

E.coli

References

Grote A， Hiller K， Scheer M et.al. JCat: a novel tool to adapt codon usage of a target gene to its potential expression host[J]. Nucleicacids Research, JUL 1 2005